Single-Molecule Förster Resonance Energy Transfer (FRET)

smFRET is a powerful biophysical technique which can reveal spatial information about biomolecules in a way which is sensitive to both dynamic and static heterogeneity between subpopulations within the same sample. It can measure kinetics of conformational changes at equilibrium with no need for synchronisation, and can measure absolute distances for infering precise structural information.

Due to the extensive use of green and red for donor/acceptor on this page, a COLOUR BLIND FRIENDLY VERSION has been made available.

Förster Resonance Energy Transfer

Confocal Single-Molecule Detection and Alternating Laser Excitation (ALEX)

Accurate FRET